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我是python领域的新手,但我想用biopython来对齐一些DNA序列。我从网上一个剧本,我跑了如下(〜是短于我的主路径):biopython的肌肉非零返回码137
~$ python
Python 3.5.1 (default, Jul 3 2016, 12:57:35)
[GCC 5.4.0 20160609] on linux
Type "help", "copyright", "credits" or "license" for more information.
>>> from Bio.Align.Applications import MuscleCommandline
>>> muscle_exe = r"/usr/bin/muscle"
>>> in_file = r"~/SpiderOak Hive/LAB/Lab book/Ery/Seqs/tester.fasta"
>>> out_file = "~/SpiderOak Hive/LAB/Lab book/Ery/Seqs/tester_aligned.fasta"
>>> muscle_cline = MuscleCommandline(muscle_exe, input=in_file, out=out_file)
>>> print(muscle_cline)
/usr/bin/muscle -in "~/SpiderOak Hive/LAB/Lab
book/Ery/Seqs/tester.fasta" -out "~/SpiderOak Hive/LAB/Lab
book/Ery/Seqs/tester_aligned.fasta"
但空空,我得到了应用程序时:
>>> muscle_cline()
Traceback (most recent call last):
File "<stdin>", line 1, in <module>
File "/opt/python3.5/lib/python3.5/site-packages/Bio/Application/__init__.py",
line 516, in __call__
stdout_str, stderr_str)
Bio.Application.ApplicationError: Non-zero return code 137 from
'/usr/bin/muscle -in "/home/gigiux/SpiderOak Hive/LAB/Lab
book/Ery/Seqs/tester.fasta" -out "/home/gigiux/SpiderOak Hive/LAB/Lab
book/Ery/Seqs/tester_aligned.fasta"', message 'MUSCLE v3.8.31 by
Robert C. Edgar'
>>>
,会是什么问题?我在网上看到,这可能是由于内存问题;在那种情况下代码是好的?我如何运行大对齐?
还有:为什么我应该使用= r“path”而不是简单的“path”?
非常感谢,
路易吉
的'r'path''问题是http://stackoverflow.com/questions/19065115/python-windows-path-slash - 在问之前你谷歌吗? – tripleee
错误消息明确指出'/ usr/bin/muscle'失败。这对你的Python代码来说不是问题,因为它可能包含无效的假设。查看'肌肉'文档以找出它失败的原因 - 无论如何,如果没有访问失败的代码,我们无法辨别它的正确与否。 – tripleee